Superscript Iv First-strand Synthesis System

Component 20-µL rxn Custom Final Conc. If gene-specific priming fails in RT-PCR repeat first-strand synthesis using oligodT as the primer. RNA targets from 100 bp to 12 kb can be detected with this system. Components SuperScript II RT 5X First-Strand Buffer 250 mM Tris-HCl pH 83 at room temperature. First-strand synthesis can be primed with the PCR primer that hybridizes nearest to the 3 terminus of the mRNA. SuperScript IV RT is available is several formats. Superior cDNA synthesis performance with even the most challenging RNA samples - Shop now.

2 rows The SuperScript IV First-Strand Synthesis System with ezDNase Enzyme for RT-PCR is optimized for.

11146016 were used according to their standard protocols unless otherwise specified. Important guidelines Pre-warm the 5 SSIV Buffer to room temperature before use. All necessary components are in separate tubes for maximum optimization of cDNA synthesis and to enable success for every. SuperScript IV First-Strand Synthesis System Components. Component 20-µL rxn Custom Final Conc. Coli RNase H total HeLa RNA sense control primer antisense control.

Superscript Iv First Strand Synthesis System

Superscript iv first-strand synthesis system. Vortex and briefly centrifuge the buffer prior to preparing the reverse transcription reaction mix. Assemble reaction mix D. The system can be used with as little as 1 ng or as much as 5 µg of total RNA.

Ad Extreme flexibility - customize the RT setup to fit the needs of your application. Note that some GSPs fail to prime cDNA synthesis even though they work in PCR on DNA templates. 11146016 were used according to their standard protocols unless otherwise specified.

It can generate cDNA up to 123 kb. Add reaction mix to annealed RNA RT reaction setup Use the measurements below to prepare your RT reaction or enter your own parameters in the column provided. After synthesis the cDNA can be amplified with specific.

SuperScript IV First-Strand Synthesis System Components. The first-strand synthesis system. SuperScript IV First-Strand Synthesis System Protocol outline A50 reactions Anneal primer to RNA B.

The following protocol is intended as a downstream application for our Purification of RNA from a DNARNA Extract protocol. SuperScript IV Reverse Transcriptase is available as stand-alone enzyme or in a first-strand synthesis system format. Synthesize first-strand cDNA at higher temperatures than conventional M-MLV RT providing increased specificity higher yields of cDNA and more full-length product.

Invitrogen SuperScript IV VILO Master Mix is a first-strand cDNA synthesis reaction mix for two-step RT-qPCR. Coli RNase H total HeLa RNA sense control primer antisense control. 2 rows The SuperScript IV First-Strand Synthesis System with ezDNase Enzyme for RT-PCR is optimized for.

The kit includes SuperScript IV Reverse Transcriptase RT a proprietary MMLV mutant that produces robust and reliable cDNA synthesis in RT reactions. The master mix format elevates the trusted VILO technology Variable Input Linear Output to the next level by combining further optimized buffer conditions with highly processive and thermostable Invitrogen SuperScript IV Reverse Transcriptase RT.

Superscript Iv First Strand Synthesis System With Ezdnase Enzyme

Invitrogen Superscript Iv First Strand Synthesis System With Ezdnase Enzyme 200 Reactions Invitrogen Superscript Iv First Strand Synthesis System With Ezdnase Enzyme Fisher Scientific

Invitrogen Superscript Iv Reverse Transcriptase 2 000 Units Invitrogen Superscript Iv Reverse Transcriptase Fisher Scientific

Superscript First Strand Synthesis System For Rt Pcr

Invitrogen Superscript Iv First Strand Synthesis System 50 Rxns Invitrogen Superscript Iv First Strand Synthesis System Fisher Scientific

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Superscript Iii First Strand Synthesis System

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This protocol describes how to synthesise a first-st.

50 reactions Store at -20C Description The SuperScript First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified polyA or total as 5 µg of total RNA. It can generate cDNA up to 123 kb. After synthesis the cDNA can be amplified with specific. The Invitrogen SuperScript IV First-Strand Synthesis System Cat. The first-strand synthesis system includes dNTPs oligodT20 random hexamers control primers and required buffer components. SuperScript IV RT 5X SuperScript IV RT buffer RNase inhibitor random hexamer primers oligodT primers dNTPs E. 50 reactions Store at -20C Description The SuperScript First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified polyA or total as 5 µg of total RNA. The kit includes SuperScript IV Reverse Transcriptase RT a proprietary MMLV mutant that produces robust and reliable cDNA synthesis in RT reactions. The amount of starting material can vary from 1 pg5 µg of total RNA.

The SuperScript IV First-Strand Synthesis System contains all components needed for RT reactions plus an additional control gene and primers and provides the flexibility to customize the RT set-up to fit the needs of your application. Assemble reaction mix D. SuperScript IV RT is available is several formats. 50 reactions Store at -20C Description The SuperScript First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified polyA or total as 5 µg of total RNA. Optional Remove genomic DNA B. All necessary components are in separate tubes for maximum optimization of cDNA synthesis and to enable success for every. Components SuperScript II RT 5X First-Strand Buffer 250 mM Tris-HCl pH 83 at room temperature.